Liu Xian-De, Shen Yun-Gang
Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.
FEBS Lett. 2004 Jul 2;569(1-3):337-40. doi: 10.1016/j.febslet.2004.05.065.
Light could induce phosphorylation of light harvesting chlorophyll a/b binding proteins (LHCII) in Dunaliella salina and spinach thylakoid membranes. We found that neither phosphorylation was affected by glycerol, whereas treatment with NaCl significantly enhanced light-induced LHCII phosphorylation in D. salina thylakoid membranes and inhibited that in spinach. Furthermore, even in the absence of light, NaCl and several other salts induced LHCII phosphorylation in D. salina thylakoid membranes, but not in spinach thylakoid membranes. In addition, hypertonic shock induced LHCII phosphorylation in intact D. salina under dark conditions and cells adapted to different NaCl concentrations exhibited similar LHCII phosphorylation levels. Taken together, these results show for the first time that while LHCII phosphorylation of D. salina thylakoid membranes resembles that of spinach thylakoid membranes in terms of light-mediated control, the two differ with respect to NaCl sensitivity under light and dark conditions.
光可诱导盐生杜氏藻和菠菜类囊体膜中光捕获叶绿素a/b结合蛋白（LHCII）的磷酸化。我们发现，甘油对这两种植物的磷酸化均无影响，而用NaCl处理可显著增强盐生杜氏藻类囊体膜中光诱导的LHCII磷酸化，并抑制菠菜类囊体膜中的该过程。此外，即使在无光条件下，NaCl和其他几种盐也能诱导盐生杜氏藻类囊体膜中LHCII的磷酸化，但不能诱导菠菜类囊体膜中的磷酸化。另外，高渗休克在黑暗条件下可诱导完整盐生杜氏藻中LHCII的磷酸化，并且适应不同NaCl浓度的细胞表现出相似的LHCII磷酸化水平。综上所述，这些结果首次表明，虽然盐生杜氏藻类囊体膜的LHCII磷酸化在光介导的调控方面与菠菜类囊体膜相似，但在光照和黑暗条件下，二者对NaCl的敏感性存在差异。
