Garraffo H M, Spande T F, Jain P, Kaneko T, Jones T H, Blum M S, Ali T M, Snelling R R, Isbell L A, Robertson H G, Daly J W
Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0820, USA.
Rapid Commun Mass Spectrom. 2001;15(16):1409-15. doi: 10.1002/rcm.382.
Chemical ionization tandem mass spectrometry (CI-MS/MS) of alkaloids with ammonia reagent gas and collision-activated dissociation as well as EI-MS/MS were applied to the tetraponerine alkaloids in extracts from six pseudomyrmecine ants of the genus Tetraponera. The MS/MS techniques along with gas chromatography Fourier transform infrared (GC/FTIR) spectra allowed identification in two extracts of seven of the eight known tetraponerines. The EI-MS/MS fragmentations proved diagnostic for the ring system and the CI-MS/MS patterns for the C-8 or C-9 substitution, while the Bohlmann bands in FTIR spectra were diagnostic for the C-8 or C-9 configurations. An Indian ant (T. allaborans) had T-2, T-4 and T-8, while a Chinese ant (T. binghami) had T-5, T-6, T-7 and T-8. Four other ants, T. rufonigra (India), T. penzigi (Africa), T. clypeata (Africa) and T. sp. cf. emeryi (Africa), had no tetraponerines.
采用以氨气为反应气的化学电离串联质谱法（CI-MS/MS）、碰撞诱导解离以及电子轰击串联质谱法（EI-MS/MS），对来自伪切叶蚁属6种伪切叶蚁提取物中的四节叶蚁生物碱进行分析。质谱联用技术以及气相色谱傅里叶变换红外光谱（GC/FTIR）可用于鉴定8种已知四节叶蚁生物碱中7种生物碱的两种提取物。EI-MS/MS碎片模式可用于鉴定环系结构，CI-MS/MS模式可用于鉴定C-8或C-9位取代情况，而FTIR光谱中的Bohlmann带可用于鉴定C-8或C-9位构型。一种印度蚂蚁（T. allaborans）含有T-2、T-4和T-8，而一种中国蚂蚁（T. binghami）含有T-5、T-6、T-7和T-8。另外4种蚂蚁，即印度的红褐四节叶蚁（T. rufonigra）、非洲的彭氏四节叶蚁（T. penzigi）、非洲的圆盾四节叶蚁（T. clypeata）以及疑似埃氏四节叶蚁（T. sp. cf. emeryi，非洲），均不含四节叶蚁生物碱。
