Christen Philippe, Bieri Stefan, Muñoz Orlando
Laboratory of Pharmacognosy and Phytochemistry, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 30 Quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
Nat Prod Commun. 2009 Oct;4(10):1341-8.
A purified stem bark extract from Schizanthus grahamii was analyzed by combining capillary gas chromatography with a selective nitrogen phosphorus detector, mass spectrometry and Fourier transform infrared spectroscopy. Several positional and configurational tropane isomers were detected and their structural elucidation was tentatively determined based on mass spectra and confirmed by vapor phase infrared spectra. Electron impact mass spectra of the isomeric alkaloids were virtually superimposable, whereas the corresponding infrared spectra differed markedly. Retention indices were established under isothermal and temperature program conditions for further peak assignment and identification. In order to point out possible artifacts in the hot and surface-active injection port, different sample introduction techniques were evaluated, namely split, splitless and on-column injections.
采用毛细管气相色谱-选择性氮磷检测器、质谱和傅里叶变换红外光谱联用技术，对格拉汉姆酸浆草纯化的茎皮提取物进行了分析。检测到了几种位置和构型的托烷异构体，并根据质谱初步确定了它们的结构，气相红外光谱对其进行了确认。异构生物碱的电子轰击质谱几乎完全重叠，而相应的红外光谱则有明显差异。在等温及程序升温条件下确定了保留指数，以便进一步进行峰归属和鉴定。为了指出热的和表面活性进样口中可能出现的假象，对不同的进样技术进行了评估，即分流进样、不分流进样和柱上进样。
