Kaiko R F, Chatterjie N, Inturrisi C E
J Chromatogr. 1975 Jun 18;109(2):247-58. doi: 10.1016/s0021-9673(01)91796-0.
A method employing solvent extraction and gas-liquid chromatography has been developed for the quantitative determination of acetylmethadol simultaneously with its two major biotransformation products, noracetylmethadol and dinoracetylmethadol. Noracetylmethadol and dinoracetylmethadol are analyzed following their conversion to the corresponding amides. The amide structure is confirmed by the use of chemical ionization mass spectroscopy and infrared spectroscopy. The method can be used to determine the concentration of acetylmethadol and these compounds in plasma samples from acetylmethadol maintenance subjects. Methadol and normethadol do not attain neasurable plasma levels. Urine contains predominantly noracetylmethadol and dinoracetylmethadol. Evidence was also obtained for the urinary excretion of acetylmethadol, methadol and normethadol. A mean quantity equal to 28% of the administered dose was excreted in the urine of a 48-h dosing interval as acetylmethadol and metabolites.
已开发出一种采用溶剂萃取和气液色谱法的方法，用于同时定量测定醋美沙朵及其两种主要生物转化产物去乙酰美沙朵和双去乙酰美沙朵。去乙酰美沙朵和双去乙酰美沙朵在转化为相应的酰胺后进行分析。通过化学电离质谱和红外光谱确认酰胺结构。该方法可用于测定醋美沙朵维持治疗受试者血浆样品中醋美沙朵及这些化合物的浓度。美沙朵和去甲美沙朵在血浆中未达到可测量水平。尿液中主要含有去乙酰美沙朵和双去乙酰美沙朵。还获得了醋美沙朵、美沙朵和去甲美沙朵经尿液排泄的证据。在48小时给药间隔期内，尿液中排泄的醋美沙朵及其代谢产物的平均量相当于给药剂量的28%。
