用于测定生物体液中α-1-乙酰美沙多及其代谢物的提取方法和薄层色谱系统。
Extraction method and thin-layer chromatographic system for the determination of alpha-l-acetylmethadol and metabolites in biological fluids.
作者信息
Kuttab S H, North-Root H, Henderson G L
出版信息
J Chromatogr. 1976 Feb 4;117(1):193-8. doi: 10.1016/s0021-9673(00)81082-1.
An extraction method and thin-layer chromatographic (TLC) system for the determination of alpha-l-acetylmethadol and its known metabolites (methadol, noracetylmethadol, dinoracetylmethadol, normethadol, 6-acetamide-4,4-diphenyl-3-heptanol, and N-methyl-6-acetamido-4,4-diphenyl-3-heptanol) are described. The parent drug and metabolites are extracted from biological fluids with ethyl acetate and separated by TLC using silica gel plates and a developing system of ethyl acetate-methanol-water-ammonia (85:10:1:1). This system may be used to quantitatively determine levels of radiolabeled drug and metabolites by scraping the TLC plates into 3-mm zonal fractions and measuring the amount of radioactivity by scintillation counting. A representative radiochromatogram obtained from an extract of monkey urine is shown.
本文描述了一种用于测定α-1-乙酰美沙多及其已知代谢物（美沙多、去甲乙酰美沙多、双去甲乙酰美沙多、去甲美沙多、6-乙酰氨基-4,4-二苯基-3-庚醇和N-甲基-6-乙酰氨基-4,4-二苯基-3-庚醇）的提取方法和薄层色谱（TLC）系统。母体药物及其代谢物用乙酸乙酯从生物流体中提取出来，并使用硅胶板和乙酸乙酯-甲醇-水-氨水（85:10:1:1）的展开系统通过TLC进行分离。该系统可用于通过将TLC板刮成3毫米的区域馏分并通过闪烁计数测量放射性量来定量测定放射性标记药物及其代谢物的水平。展示了从猴尿提取物中获得的代表性放射色谱图。
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