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[液体培养基中结核分枝杆菌培养测定的自动化方法]

[Automated methods of culture determination of M. tuberculosis in liquid media].

作者信息

Irtuganova O A, Smirnova N S, Slogotskaia L V, Moroz A M, Litvinov V I

出版信息

Probl Tuberk. 2001(3):53-5.

PMID:11508237
Abstract

A hundred and seventy respiratory samples from patients with different forms of tuberculosis were used to test the efficiency of the automatic liquid culture systems BACTEC MGIT 960 and MB/BacT with inoculation into the standard dense media. All these media provided 47 M. tuberculous isolates, of them 41 (87.2%), 38 (80.9%), and 76.6% on the BACTER 960, MB/BacT, and dense media, respectively. The average time of detection of mycobacterial growth by means of automatic systems was much shorter and equal to 10.7 days on the BACTEC 960 and 18.7 days on the MB/BacT versus 33.2 days on the standard dense medium. In terms of their sensitivity and detection rate, the automatic systems were superior to the dense media widely used in laboratory practice.

摘要

从患有不同类型肺结核的患者中采集了170份呼吸道样本,用于测试自动液体培养系统BACTEC MGIT 960和MB/BacT接种到标准致密培养基中的效率。所有这些培养基共培养出47株结核分枝杆菌分离株,其中在BACTER 960、MB/BacT和致密培养基上分别培养出41株(87.2%)、38株(80.9%)和76.6%。通过自动系统检测分枝杆菌生长的平均时间要短得多,在BACTEC 960上为10.7天,在MB/BacT上为18.7天,而在标准致密培养基上为33.2天。就敏感性和检测率而言,自动系统优于实验室实践中广泛使用的致密培养基。

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引用本文的文献

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Meta-analysis of BACTEC MGIT 960 and BACTEC 460 TB, with or without solid media, for detection of mycobacteria.对BACTEC MGIT 960和BACTEC 460结核分枝杆菌检测方法(无论有无固体培养基)进行的荟萃分析。
J Clin Microbiol. 2004 May;42(5):2321-5. doi: 10.1128/JCM.42.5.2321-2325.2004.