Inukai T, Takanashi K, Takebayashi K, Tayama K, Aso Y, Takiguchi Y, Takemura Y
Department of Medicine, Koshigaya Hospital, Dokkyo University School of Medicine, Minami-Koshigaya, Japan.
J Med. 2000;31(5-6):247-61.
We investigated the change in low-density lipoprotein receptor activity following the administration of estrogen to patients with hypercholesterolemia (HC). Studies were conducted in 16 patients with HC (total cholesterol (TC)>220 mg/dL) and 133 healthy control subjects. LDL-R activity was measured by fluorocytometry. Three of sixteen patients with HC showed low LDL-R activity below 80% together with extremely high serum levels of TC and LDL cholesterol (LDL-C). LDL-R activity showed an inverse correlation with serum levels of TC and LDL-C (p<0.05, respectively). Two women with initially low LDL-R activity who showed a marked increase in LDL-R activity exhibited a normalized activity at four and eight weeks after estriol administration, proportional to the reduction in serum levels of TC and LDL-C. One man with an initially extremely low LDL-R activity showed no abnormality at the sites of exons 7, 14, 17 and intron 12 by the PCR-DGGE method, which are common sites for point-mutations of LDL-R among Japanese patients with HC. Estrogen reduced serum levels of TC and LDL-C in patients with HC, at least in part, via an increase in the LDL-R activity of patients with HC and an initially low LDL-R activity.
我们研究了向高胆固醇血症（HC）患者施用雌激素后低密度脂蛋白受体活性的变化。对16例HC患者（总胆固醇（TC）>220mg/dL）和133名健康对照者进行了研究。通过荧光细胞术测量低密度脂蛋白受体（LDL-R）活性。16例HC患者中有3例LDL-R活性低于80%，同时血清TC和低密度脂蛋白胆固醇（LDL-C）水平极高。LDL-R活性与血清TC和LDL-C水平呈负相关（p均<0.05）。两名初始LDL-R活性较低的女性在施用雌三醇后4周和8周时LDL-R活性显著增加，活性恢复正常，这与血清TC和LDL-C水平的降低成比例。一名初始LDL-R活性极低的男性通过PCR-DGGE方法检测发现其第7、14、17外显子和第12内含子位点无异常，这些位点是日本HC患者中LDL-R点突变的常见位点。雌激素至少部分地通过增加HC患者以及初始LDL-R活性较低患者的LDL-R活性，降低了HC患者的血清TC和LDL-C水平。
