Nakagawasai O, Tadano T, Hozumi S, Taniguchi R, Tan-No K, Esashi A, Niijima F, Kisara K
Department of Pharmacology, Tohoku Pharmaceutical University, Sendai, Japan.
Life Sci. 2001 Jul 27;69(10):1181-91. doi: 10.1016/s0024-3205(01)01206-1.
We produced thiamine-deficient (TD) mice by TD diet treatment. The growth curve of mice on TD feeding was sharply increased until on the 10th day and subsequently the body weight gradually decreased. The mortality rate in mice was about 67% on the 30th day after the start of TD feeding. We performed the forced swimming test on the 10th and 20th day after the start of TD feeding. The duration of immobility in the forced swimming test was increased on the 20th day of TD feeding. Locomotor activity and motor co-ordination between the pair-fed control group and TD group on the 20th day of TD feeding were not significantly changed. Only a single injection of thiamine HCI (50 mg/kg, s.c.) on the 10th day after the start of a TD diet shortened the increased duration of immobility in the forced swimming test on the 20th day after the start of TD feeding. Whereas these reversal effects of thiamine treatment on the 20th day were not found when the treatment was given on the 19th day after the start of a TD diet. On the 20th day after the start of TD feeding, the increased duration of immobility time induced by TD was shortened by chronic administration of the tricyclic antidepressant imipramine (10 mg/kg, i.p.). These results suggested that behavioral changes in the forced swimming test might be involved in the degeneration of serotonergic and noradrenergic neurons.
我们通过给予硫胺素缺乏（TD）饮食来培育TD小鼠。TD喂养的小鼠生长曲线在第10天之前急剧上升，随后体重逐渐下降。TD喂养开始后第30天，小鼠的死亡率约为67%。我们在TD喂养开始后的第10天和第20天进行了强迫游泳试验。TD喂养第20天时，强迫游泳试验中的不动时间增加。在TD喂养第20天时，配对喂养对照组和TD组之间的运动活动和运动协调性没有显著变化。在TD饮食开始后的第10天单次注射盐酸硫胺素（50mg/kg，皮下注射），可缩短TD喂养开始后第20天强迫游泳试验中增加的不动时间。然而，当在TD饮食开始后的第19天进行治疗时，未发现硫胺素治疗在第20天的这些逆转作用。在TD喂养开始后的第20天，慢性给予三环类抗抑郁药丙咪嗪（10mg/kg，腹腔注射）可缩短TD诱导的不动时间增加的时长。这些结果表明，强迫游泳试验中的行为变化可能与5-羟色胺能和去甲肾上腺素能神经元的退化有关。
