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非洲爪蟾U3小核仁RNA的GAC盒A'和A盒序列在核糖体RNA加工中发挥不同的功能作用。

Xenopus U3 snoRNA GAC-Box A' and Box A sequences play distinct functional roles in rRNA processing.

作者信息

Borovjagin A V, Gerbi S A

机构信息

Division of Biology and Medicine, Brown University, Providence, Rhode Island 02912, USA.

出版信息

Mol Cell Biol. 2001 Sep;21(18):6210-21. doi: 10.1128/MCB.21.18.6210-6221.2001.

DOI:10.1128/MCB.21.18.6210-6221.2001
PMID:11509664
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC87338/
Abstract

Mutations in the 5' portion of Xenopus U3 snoRNA were tested for function in oocytes. The results revealed a new cleavage site (A0) in the 3' region of vertebrate external transcribed spacer sequences. In addition, U3 mutagenesis uncoupled cleavage at sites 1 and 2, flanking the 5' and 3' ends of 18S rRNA, and generated novel intermediates: 19S and 18.5S pre-rRNAs. Furthermore, specific nucleotides in Xenopus U3 snoRNA that are required for cleavages in pre-rRNA were identified: box A is essential for site A0 cleavage, the GAC-box A' region is necessary for site 1 cleavage, and the 3' end of box A' and flanking nucleotides are required for site 2 cleavage. Differences between metazoan and yeast U3 snoRNA-mediated rRNA processing are enumerated. The data support a model where metazoan U3 snoRNA acts as a bridge to draw together the 5' and 3' ends of the 18S rRNA coding region within pre-rRNA to coordinate their cleavage.

摘要

对非洲爪蟾U3小核仁RNA（snoRNA）5'端的突变进行了卵母细胞中的功能测试。结果揭示了脊椎动物外部转录间隔序列3'区域中的一个新的切割位点（A0）。此外，U3诱变使18S rRNA 5'和3'端两侧的位点1和位点2的切割解偶联，并产生了新的中间体：19S和18.5S前体rRNA。此外，还鉴定了非洲爪蟾U3 snoRNA中前体rRNA切割所需的特定核苷酸：A框对于A0位点切割至关重要，GAC - A'框区域对于位点1切割是必需的，A'框的3'端和侧翼核苷酸对于位点2切割是必需的。列举了后生动物和酵母U3 snoRNA介导的rRNA加工之间的差异。数据支持一种模型，即后生动物U3 snoRNA作为桥梁，将前体rRNA中18S rRNA编码区域的5'和3'端聚集在一起，以协调它们的切割。

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