运用双同位素放射自显影术对小鼠脾细胞摄取[3H]胸腺嘧啶核苷、[131I]5-碘-2'-脱氧尿苷及[3H]脱氧胞苷的体外情况进行的比较研究。

A comparative study on the uptake in vitro of [3H]thymidine, [131I]5-iodo-2'-deoxyuridine and [3H]deoxycytidine in mouse spleen cells using double isotope autoradiography.

作者信息

Rooijen N V

出版信息

J Immunol Methods. 1975;8(1-2):151-7. doi: 10.1016/0022-1759(75)90091-5.

Abstract

The uptake of 3H-labeled thymidine (3H-TdR), 131I-labelled 5-iodo-2'-deoxyuridine (131IUdR) and 3H-labelled deoxycytidine (3H-CdR) by mouse spleen cells in vitro was studied using an autoradiographic method for the separate detection of 3H and 131I in cell smears. The experiment was performed in two steps. In one half of a pooled spleen cell suspension uptake of 3H-TdR and 131IUdR was compared; in the other half of the suspension uptake of 3H-CdR and 131IUdR was compared. Only two nucleated spleen cells were found which had taken up only 3H-CdR. All other scanned cells were double labelled. Generally the nucleotides were taken up according to a rather fixed relation. It was further found that 3H-CdR and 3H-TdR were incorporated to about the same extent. However, cells which were incubated with 131IUdR and 3H-TdR took up the 131IUdR to a lesser extent than cells which were incubated with 131IUdR and 3H-CdR.

摘要

采用放射自显影法在细胞涂片上分别检测³H和¹³¹I，研究了³H标记的胸腺嘧啶核苷（³H-TdR）、¹³¹I标记的5-碘-2'-脱氧尿苷（¹³¹IUdR）和³H标记的脱氧胞苷（³H-CdR）被小鼠脾细胞体外摄取的情况。实验分两步进行。在合并的脾细胞悬液的一半中比较³H-TdR和¹³¹IUdR的摄取；在悬液的另一半中比较³H-CdR和¹³¹IUdR的摄取。仅发现两个有核脾细胞只摄取了³H-CdR。所有其他扫描的细胞都是双重标记的。一般来说，核苷酸是按照相当固定的比例被摄取的。进一步发现³H-CdR和³H-TdR的掺入程度大致相同。然而，与¹³¹IUdR和³H-TdR一起孵育的细胞摄取¹³¹IUdR的程度低于与¹³¹IUdR和³H-CdR一起孵育的细胞。