Shou W, Sakamoto K M, Keener J, Morimoto K W, Traverso E E, Azzam R, Hoppe G J, Feldman R M, DeModena J, Moazed D, Charbonneau H, Nomura M, Deshaies R J
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Mol Cell. 2001 Jul;8(1):45-55. doi: 10.1016/s1097-2765(01)00291-x.
The budding yeast RENT complex, consisting of at least three proteins (Net1, Cdc14, Sir2), is anchored to the nucleolus by Net1. RENT controls mitotic exit, nucleolar silencing, and nucleolar localization of Nop1. Here, we report two new functions of Net1. First, Net1 directly binds Pol I and stimulates rRNA synthesis both in vitro and in vivo. Second, Net1 modulates nucleolar structure by regulating rDNA morphology and proper localization of multiple nucleolar antigens, including Pol I. Importantly, we show that the nucleolar and previously described cell cycle functions of the RENT complex can be uncoupled by a dominant mutant allele of CDC14. The independent functions of Net1 link a key event in the cell cycle to nucleolar processes that are fundamental to cell growth.
出芽酵母RENT复合体由至少三种蛋白质（Net1、Cdc14、Sir2）组成，通过Net1锚定在核仁上。RENT控制有丝分裂退出、核仁沉默以及Nop1的核仁定位。在此，我们报告Net1的两个新功能。首先，Net1直接结合RNA聚合酶I（Pol I）并在体外和体内刺激rRNA合成。其次，Net1通过调节rDNA形态和多种核仁抗原（包括Pol I）的正确定位来调节核仁结构。重要的是，我们表明RENT复合体的核仁功能和先前描述的细胞周期功能可被CDC14的显性突变等位基因解开。Net1的独立功能将细胞周期中的一个关键事件与对细胞生长至关重要的核仁过程联系起来。
