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Net1 的 C 端区域是 RNA 聚合酶 I 转录的激活因子，在从酵母到人中具有保守特征。

The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human.

机构信息

Institut für Biochemie, Genetik und Mikrobiologie, Universität Regensburg, Regensburg, Germany.

出版信息

PLoS Genet. 2019 Feb 25;15(2):e1008006. doi: 10.1371/journal.pgen.1008006. eCollection 2019 Feb.

DOI:10.1371/journal.pgen.1008006
PMID:30802237
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6415870/
Abstract

RNA polymerase I (Pol I) synthesizes ribosomal RNA (rRNA) in all eukaryotes, accounting for the major part of transcriptional activity in proliferating cells. Although basal Pol I transcription factors have been characterized in diverse organisms, the molecular basis of the robust rRNA production in vivo remains largely unknown. In S. cerevisiae, the multifunctional Net1 protein was reported to stimulate Pol I transcription. We found that the Pol I-stimulating function can be attributed to the very C-terminal region (CTR) of Net1. The CTR was required for normal cell growth and Pol I recruitment to rRNA genes in vivo and sufficient to promote Pol I transcription in vitro. Similarity with the acidic tail region of mammalian Pol I transcription factor UBF, which could partly functionally substitute for the CTR, suggests conserved roles for CTR-like domains in Pol I transcription from yeast to human.

摘要

RNA 聚合酶 I（Pol I）在所有真核生物中合成核糖体 RNA（rRNA），占增殖细胞中大部分转录活性。尽管在不同的生物体中已经鉴定出了基础的 Pol I 转录因子，但体内强大的 rRNA 产生的分子基础在很大程度上仍然未知。在酿酒酵母中，多功能 Net1 蛋白被报道能刺激 Pol I 转录。我们发现，Pol I 刺激功能可以归因于 Net1 的非常 C 末端区域（CTR）。CTR 是细胞正常生长和 Pol I 在体内募集到 rRNA 基因所必需的，并且足以促进 Pol I 在体外转录。与哺乳动物 Pol I 转录因子 UBF 的酸性尾巴区域的相似性，它可以部分地替代 CTR，这表明在从酵母到人类的 Pol I 转录中，CTR 样结构域具有保守的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/cf741bdc94e8/pgen.1008006.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/4016c3f00a0d/pgen.1008006.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/71e331ee1258/pgen.1008006.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/f37fe63dc69a/pgen.1008006.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/01527b27117b/pgen.1008006.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/762a7785e6fa/pgen.1008006.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/e82324a8c6b9/pgen.1008006.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/cf741bdc94e8/pgen.1008006.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/4016c3f00a0d/pgen.1008006.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/62eb31a96afd/pgen.1008006.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/71e331ee1258/pgen.1008006.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/f37fe63dc69a/pgen.1008006.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/01527b27117b/pgen.1008006.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/762a7785e6fa/pgen.1008006.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/e82324a8c6b9/pgen.1008006.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/665f/6415870/cf741bdc94e8/pgen.1008006.g008.jpg

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