酗酒导致直肠细胞增殖增加。
Increased rectal cell proliferation following alcohol abuse.
作者信息
Simanowski U A, Homann N, Knühl M, Arce L, Waldherr R, Conradt C, Bosch F X, Seitz H K
机构信息
Laboratory of Alcohol Research, Liver Disease and Nutrition, and Department of Medicine, Salem Medical Centre, Heidelberg, Germany.
出版信息
Gut. 2001 Sep;49(3):418-22. doi: 10.1136/gut.49.3.418.
BACKGROUND
Epidemiological data indicate an increased risk for rectal cancer following chronic alcohol consumption. As chronic ethanol ingestion leads to rectal hyperregeneration in experimental animals, indicating a state of increased susceptibility to carcinogens, we studied cell proliferation in alcohol abusers.
METHODS
Rectal biopsies were taken from 44 heavy drinkers and 26 controls. Cell proliferation, including proliferative compartment size, was measured by immunohistological staining for proliferative cell nuclear antigen (PCNA) and Ki67, and by in situ hybridisation for histone H3. Quantification of cell proliferation using PCNA staining was evaluated in 27 alcohol abusers and 12 controls. In addition, immunohistology was performed for cytokeratins and gene products of Rb1, bcl-2, and p53.
RESULTS
Heavy drinking resulted in increased cell proliferation of the rectal mucosa, as shown by increased detection of different proliferation markers. However, cell differentiation regarding cytokeratin expression patterns was unchanged as well as regulatory factors involved in carcinogenesis and/or apoptosis.
CONCLUSION
Chronic alcohol abuse leads to rectal mucosal hyperproliferation in humans, a condition associated with an increased cancer risk.
背景
流行病学数据表明，长期饮酒会增加患直肠癌的风险。由于在实验动物中，长期摄入乙醇会导致直肠过度再生，这表明对致癌物的易感性增加，我们对酗酒者的细胞增殖情况进行了研究。
方法
对44名重度饮酒者和26名对照者进行直肠活检。通过增殖细胞核抗原（PCNA）和Ki67的免疫组织化学染色以及组蛋白H3的原位杂交来测量细胞增殖，包括增殖区室大小。在27名酗酒者和12名对照者中，利用PCNA染色对细胞增殖进行定量评估。此外，还对细胞角蛋白以及Rb1、bcl-2和p53的基因产物进行了免疫组织学检测。
结果
重度饮酒导致直肠黏膜细胞增殖增加，不同增殖标志物的检测结果增加表明了这一点。然而，细胞角蛋白表达模式的细胞分化以及参与致癌和/或凋亡的调节因子并未改变。
结论
长期酗酒会导致人类直肠黏膜过度增殖，这种情况与癌症风险增加有关。
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