Murray S C, Sandler R S, Keku T O, Lyles C M, Millikan R C, Bangdiwala S I, Kupper L L, Jiang W, Ulshen M H
Department of Medicine, University of North Carolina at Chapel Hill 27599-7080, USA.
Cancer Epidemiol Biomarkers Prev. 1995 Oct-Nov;4(7):715-20.
Rectal mucosal proliferation has been promoted as an intermediate marker for risk of colorectal neoplasia. Proliferating cell nuclear antigen (PCNA) immunohistochemistry has become a standard method to measure cell proliferation. Whole-crypt dissection may provide a technically simpler method for determining proliferation within an entire crypt. We conducted a study to assess the reliability (reproducibility) of whole-crypt dissection in 10 subjects. Reliability of whole-crypt dissection with the subject as the unit of observation was excellent. The intraclass correlation coefficient for subjects was 0.93. Biopsy-to-biopsy reliability was lower (r=0.86) and crypt-to-crypt reliability lower still (r = 0.35). There was poor correlation between measures of proliferation index using the two techniques (Kendall's tau = 0.13; P = 0.08). Compartment analysis based on the percentage of the total number of labeled cells appearing in each crypt quartile also did not demonstrate a significant correlation between the two measures. We conclude that PCNA labeling index and whole-crypt mitotic count are not comparable measures of rectal mucosal proliferation.
直肠黏膜增殖已被视为结直肠肿瘤发生风险的一个中间标志物。增殖细胞核抗原（PCNA）免疫组化已成为测量细胞增殖的标准方法。全隐窝解剖可能为确定整个隐窝内的增殖情况提供一种技术上更简单的方法。我们开展了一项研究，以评估10名受试者全隐窝解剖的可靠性（可重复性）。以受试者作为观察单位时，全隐窝解剖的可靠性极佳。受试者的组内相关系数为0.93。活检间的可靠性较低（r = 0.86），隐窝间的可靠性更低（r = 0.35）。使用这两种技术测量的增殖指数之间相关性较差（肯德尔等级相关系数τ = 0.13；P = 0.08）。基于每个隐窝四分位数中标记细胞总数的百分比进行的区室分析也未显示这两种测量方法之间存在显著相关性。我们得出结论，PCNA标记指数和全隐窝有丝分裂计数不是直肠黏膜增殖的可比测量指标。
