Casoli T, Di Stefano G, Gracciotti N, Giovagnetti S, Fattoretti P, Solazzi M, Bertoni-Freddari C
Neurobiology of Aging, INRCA Research Department, Via Birarelli 8, Ancona AN 60121, Italy.
J Histochem Cytochem. 2001 Sep;49(9):1195-6. doi: 10.1177/002215540104900917.
The growth-associated protein GAP-43 is a presynaptic membrane phosphoprotein that plays a key role in guiding the growth of axons and in modulating the formation of new synapses. To identify the cells that synthesize GAP-43 mRNA, we applied direct in situ reverse transcription-polymerase chain reaction (in situ RT-PCR) in cerebellum and hippocampus of adult rat brain. In situ RT-PCR revealed GAP-43 mRNA in cerebellar granule cells, in Purkinje cells and in some interneurons of the molecular layer. Previous in situ hybridization studies had demonstrated a dense label throughout the granular layer of the cerebellar cortex but no labeling of other cerebellar neurons. Hippocampal cells showing distinct GAP-43 mRNA signal after in situ RT-PCR were CA1 and CA3 pyramidal neurons, CA4 hilar cells, and dentate gyrus granule cells, whereas in situ hybridization studies had detected GAP-43 mRNA only in CA3 and CA1 pyramidal neurons. Our data indicate that GAP-43 mRNA is widely distributed, suggesting that many cell types are potentially involved in synaptic plasticity events. (J Histochem Cytochem 49:1195-1196, 2001)
生长相关蛋白GAP - 43是一种突触前膜磷蛋白，在引导轴突生长和调节新突触形成中起关键作用。为了鉴定合成GAP - 43 mRNA的细胞，我们在成年大鼠脑的小脑和海马中应用了直接原位逆转录聚合酶链反应（原位RT - PCR）。原位RT - PCR显示在小脑颗粒细胞、浦肯野细胞和分子层的一些中间神经元中有GAP - 43 mRNA。先前的原位杂交研究表明，在小脑皮质的整个颗粒层有密集标记，但其他小脑神经元无标记。原位RT - PCR后显示出明显GAP - 43 mRNA信号的海马细胞是CA1和CA3锥体神经元、CA4门区细胞和齿状回颗粒细胞，而原位杂交研究仅在CA3和CA1锥体神经元中检测到GAP - 43 mRNA。我们的数据表明GAP - 43 mRNA分布广泛，提示许多细胞类型可能参与突触可塑性事件。（《组织化学与细胞化学杂志》49:1195 - 1196，2001）
