Atkinson H J
J Exp Biol. 1975 Feb;62(1):1-9. doi: 10.1242/jeb.62.1.1.
An animal chamber and a simple microspectrophotometer for investigating the in vivo oxygenation of the haemoglobin of E. brevis are described. The in vivo absorption peaks of this haemoglobin occur at similar wavelengths to those of other nematodes. Mean values, given with their corresponding standard errors, occur at 577.6 plus or minus 0.6 nm, 543.6 plus or minus 0.5 nm and 421.7 plus or minus 1.9 nm for oxyhaemoglobin, and 555.2 plus or minus 0.9 nm and 432.2 plus or minus 1.3 nm for the deoxygenated pigment. The percentage of oxyhaemoglobin in the pharynx of E. brevis decreased at external oxygen tensions of less than 20 Torr, and the pigment was completely deoxygenated at 5 Torr. Stimulation of individuals in aerated sea water for 1-2 min caused a partial deoxygenation of the haemoglobin; the pigment reloaded soon after this period of increased activity had ended. The functional significance of the haemoglobin of E. brevis is disucssed.
描述了一种用于研究短尾艾氏线虫血红蛋白体内氧合作用的动物饲养室和一台简易显微分光光度计。这种血红蛋白的体内吸收峰出现在与其他线虫相似的波长处。给出了氧合血红蛋白的平均值及其相应标准误差，分别为577.6±0.6纳米、543.6±0.5纳米和421.7±1.9纳米；脱氧色素的平均值及其相应标准误差分别为555.2±0.9纳米和432.2±1.3纳米。当外部氧张力低于20托时，短尾艾氏线虫咽部的氧合血红蛋白百分比下降，在5托时色素完全脱氧。在曝气海水中刺激个体1 - 2分钟会导致血红蛋白部分脱氧；在这段活动增加期结束后不久，色素又重新充氧。文中讨论了短尾艾氏线虫血红蛋白的功能意义。
