Devogel M, Léonis J, Vincentelli J
Experientia. 1977 Nov 15;33(11):1429-50. doi: 10.1007/BF01918785.
Kinetic investigation by means of stopped flow techniques showed the rate of deoxygenation of haemoglobin to be slower in the presence of spectrin. At pH 7.15, the kinetic constant was 27.2 sec-1 in presence of spectrin instead of 34.3 sec-1 for haemoglobin alone. Also, equilibrium studies have revealed that the oxygen pressure for half-saturated haemoglobin decreased when spectrin was added to the reaction medium. At pH 7.35, the log (pO2)1/2 was 0.88 for haemoglobin in presence of spectrin instead of 0.93 for haemoglobin alone. From these results, an interaction between spectrin and haemoglobin may be suspected.
通过停流技术进行的动力学研究表明，在血影蛋白存在的情况下，血红蛋白的脱氧速率较慢。在pH 7.15时，在血影蛋白存在下的动力学常数为27.2秒-1，而单独的血红蛋白为34.3秒-1。此外，平衡研究表明，当向反应介质中添加血影蛋白时，半饱和血红蛋白的氧分压降低。在pH 7.35时，在血影蛋白存在下血红蛋白的log (pO2)1/2为0.88，而单独的血红蛋白为0.93。从这些结果可以推测血影蛋白和血红蛋白之间存在相互作用。
