Arlotto T, Gerber D, Terblanche S J, Larsen J
Department of Reproduction, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
J S Afr Vet Assoc. 2001 Jun;72(2):72-5. doi: 10.4102/jsava.v72i2.620.
In vitro fertilisation (IVF) has become a useful breeding tool in most of the developed world. In this paper the success of bovine IVF and the birth of live calves under typical South African conditions is reported. Oocytes for IVF were collected from the ovaries of 6 slaughtered Bovelder beef cows. On average, 36.2 oocytes per donor were retrieved. From these oocytes, 43 blastocysts were produced from 5 of the donors by IVF with frozen Bovelder semen. The best 11 of these embryos were transferred into oestrous, synchronised Bovelder recipients in the same herd. As a result, 7 calves were born (a 64% calving rate) from 4 of the original donors. The calves had a normal birth mass, but the mean gestation length of the male calves was significantly longer than the herd average (291.6 versus 285.2 days respectively). No calving difficulties were encountered. In summary, it was shown that IVF for bovine embryo production and transfer is possible on a commercial basis in South Africa.
体外受精（IVF）在大多数发达国家已成为一种有用的繁殖工具。本文报道了在典型的南非条件下牛体外受精的成功及活牛犊的出生情况。用于体外受精的卵母细胞从6头被屠宰的博尔德肉牛的卵巢中采集。每个供体平均采集到36.2个卵母细胞。从这些卵母细胞中，使用冷冻的博尔德精液通过体外受精，5个供体产生了43个囊胚。其中最好的11个胚胎被移植到同一牛群中处于发情期且同期发情的博尔德受体母牛体内。结果，来自4个原始供体的7头小牛出生（产犊率为64%）。这些小牛出生体重正常，但雄性小牛的平均妊娠期明显长于牛群平均水平（分别为291.6天和285.2天）。未遇到产犊困难。总之，结果表明在南非，牛胚胎生产和移植的体外受精在商业基础上是可行的。
