Woods D R, Thomson J A
J Gen Microbiol. 1975 May;88(1):86-92. doi: 10.1099/00221287-88-1-86.
Six auxotrophic markers of a halotolerant collagenolytic strain of Achromobacter were transduced by four alpha hages. Abortive transduction was also demonstrated. The generalized transduction system is unusual as the transductants were unstable, characteristic of transduction by lysogeny. The Achromobacter strain is a cryptic lysogen for alpha and purified transductants were either sensitive or resistant to alpha. Purified clones from four resistant transductants and one sensitive transductant liberated phage spontaneously. The host ranges of these spontaneous phage differed from that of the alpha phage used for the transduction experiment. Some initially resistant transductants became simi-sensitive to alpha (efficiency to plating) e.o.p. (10minus-1 to 10minus-2) after repeated cloning.
无色杆菌的一株耐盐性胶原分解菌株的六个营养缺陷型标记被四种α噬菌体转导。还证实了流产转导。这种普遍转导系统很不寻常，因为转导子不稳定，这是溶源化转导的特征。无色杆菌菌株是α的隐性溶源菌，纯化的转导子对α要么敏感要么抗性。来自四个抗性转导子和一个敏感转导子的纯化克隆自发释放噬菌体。这些自发噬菌体的宿主范围与用于转导实验的α噬菌体不同。一些最初抗性的转导子在反复克隆后对α（平板接种效率）变得类似敏感（效价为10的负1次方至10的负2次方）。
