Monteiro S, Piçarra-Pereira M A, Mesquita P R, Loureiro V B, Teixeira A, Ferreira R B
Instituto Superior de Agronomia, Universidade Técnica de Lisboa, 1349-017 Lisboa, Portugal.
J Agric Food Chem. 2001 Aug;49(8):3999-4010. doi: 10.1021/jf0013914.
In the present work, single grape variety wines, Moscatel and Arinto, were used. Analysis by denaturing polyacrylamide gel electrophoresis of the wine proteins revealed the presence of only a few polypeptides ranging in molecular mass from 15 to 30 kDa. However, a more detailed examination of the whole protein fraction, by a combination of techniques, showed that these wines contain a very large number (many tens and, possibly, many more) of distinct polypeptides, exhibiting similar molecular masses but different electrical charges. The results obtained using highly specific antibodies and N-terminal sequencing indicate that there is structural similarity among most of the wine polypeptides. These observations can be explained by the existence of a common precursor to most or all of the wine proteins, which could generate all of the detected polypeptides by limited proteolysis. Comparison of the N-terminal sequences of the polypeptides isolated from Moscatel wine with proteins from other sources revealed a high degree of homology to pathogenesis-related proteins.
在本研究中，使用了单一葡萄品种葡萄酒，即麝香葡萄酒和阿林托葡萄酒。通过对葡萄酒蛋白质进行变性聚丙烯酰胺凝胶电泳分析，结果显示仅存在少数分子量在15至30 kDa之间的多肽。然而，通过多种技术相结合对整个蛋白质组分进行更详细的检查发现，这些葡萄酒含有大量（数十种甚至可能更多）不同的多肽，它们具有相似的分子量但不同的电荷。使用高度特异性抗体和N端测序获得的结果表明，大多数葡萄酒多肽之间存在结构相似性。这些观察结果可以通过大多数或所有葡萄酒蛋白质存在共同前体来解释，该前体可通过有限的蛋白水解产生所有检测到的多肽。将从麝香葡萄酒中分离出的多肽的N端序列与其他来源的蛋白质进行比较，发现与病程相关蛋白具有高度同源性。
