一项关于人类线粒体RNA聚合酶活性的研究表明存在一种类转录因子B蛋白。
A study on the human mitochondrial RNA polymerase activity points to existence of a transcription factor B-like protein.
作者信息
Prieto-Martín A, Montoya J, Martínez-Azorín F
机构信息
Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Miguel Servet 177, 50013, Zaragoza, Spain.
出版信息
FEBS Lett. 2001 Aug 10;503(1):51-5. doi: 10.1016/s0014-5793(01)02695-3.
In the present work, the RNA polymerase activity of the human mitochondrial RNA polymerase mature protein (h-mtRPOLm) is shown, and its molecular activity calculated (2.1+/-0.9 min(-1)). An activity analysis of h-mtRPOLm and deleted versions of it has demonstrated that the entire recombinant protein is required for this activity. In addition, h-mtRPOLm alone or in presence of the known mitochondrial transcription factors (human mitochondrial transcription factor A and/or human mitochondrial transcription termination factor) is not able to initiate transcription from the specific human mitochondrial promoters pointing to the existence of a human mitochondrial transcription factor B-like protein.
在本研究中，展示了人类线粒体RNA聚合酶成熟蛋白（h-mtRPOLm）的RNA聚合酶活性，并计算了其分子活性（2.1±0.9 min⁻¹）。对h-mtRPOLm及其缺失版本的活性分析表明，该活性需要完整的重组蛋白。此外，单独的h-mtRPOLm或在已知线粒体转录因子（人类线粒体转录因子A和/或人类线粒体转录终止因子）存在的情况下，都无法从特定的人类线粒体启动子起始转录，这表明存在一种类似人类线粒体转录因子B的蛋白。
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