委内瑞拉尤卡帕印第安人中丁型肝炎病毒基因型III的特征分析。

Characterization of hepatitis D virus genotype III among Yucpa Indians in Venezuela.

作者信息

Nakano Tatsunori, Shapiro Craig N, Hadler Stephan C, Casey John L, Mizokami Masashi, Orito Etsuro, Robertson Betty H

机构信息

Second Department of Medicine, Nagoya City University Medical School, Nagoya 467-8601, Japan3.

Division of Viral Hepatitis, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA1.

出版信息

J Gen Virol. 2001 Sep;82(Pt 9):2183-2189. doi: 10.1099/0022-1317-82-9-2183.

DOI:10.1099/0022-1317-82-9-2183

PMID:11514728

Abstract

The complete genome sequences of hepatitis D virus (HDV) strains isolated from three Yucpa Amerindians in Venezuela were determined and found to be genotype III. Comparison of these three genotype III sequences demonstrated the presence of a hypervariable region containing numerous substitutions, insertions/deletions and a highly conserved region containing the self-cleavage domains, which have been reported previously for genotypes I and II. Amino acid changes within the first 90 amino acids of the hepatitis D antigen (HDAg) were found in the genotype III sequences, while the remainder of the HDAg-coding sequence was conserved. The secondary structure for the RNA-editing site differed between genotypes I and III. It was concluded that the serious delta hepatitis outbreaks characterized epidemiologically in the Yucpa Amerindians were caused by HDV genotype III isolates that were related to HDV genotype III isolates from other regions of South America.

摘要

对从委内瑞拉的三名尤卡帕美洲印第安人身上分离出的丁型肝炎病毒（HDV）毒株的全基因组序列进行了测定，发现其为III型基因型。对这三个III型基因型序列的比较表明，存在一个包含众多替换、插入/缺失的高变区以及一个包含自我切割结构域的高度保守区，这在之前已报道的I型和II型基因型中也有发现。在III型基因型序列中发现了丁型肝炎抗原（HDAg）前90个氨基酸内的氨基酸变化，而HDAg编码序列的其余部分是保守的。I型和III型基因型之间RNA编辑位点的二级结构有所不同。得出的结论是，尤卡帕美洲印第安人在流行病学上表现出的严重丁型肝炎暴发是由与来自南美洲其他地区的HDV III型基因型毒株相关的HDV III型基因型分离株引起的。