Lin Guangyun, Slack Jeffrey M, Blissard Gary W
Boyce Thompson Institute at Cornell University, Tower Road, Ithaca, NY 14853-1801, USA1.
J Gen Virol. 2001 Sep;82(Pt 9):2289-2294. doi: 10.1099/0022-1317-82-9-2289.
The Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) lef-11 gene was found previously to be necessary to support optimal levels of transient expression from an AcMNPV late promoter. The lef-11 gene is unusual in that it overlaps both upstream (orf38) and downstream (pp31) genes. In this study, the expression and cellular localization of LEF-11 were examined. The lef-11 transcripts were detected from 4 to 36 h post-infection (p.i.). The 1.5 kb lef-11 mRNA initiates 196 nt upstream of the lef-11 translation initiation codon, within the upstream orf38 gene. This relatively long 5' upstream region encodes a potential small upstream open reading frame (ORF) of 58 amino acids that overlaps the lef-11 ORF. The 3' end of the lef-11 mRNA was mapped as co-terminal with mRNAs from the downstream pp31 gene. Using affinity purified anti-LEF-11 antibodies, levels of LEF-11 expression were found to be maximal between approximately 8 and 24 h p.i., although LEF-11 could be detected as late as 72 h p.i. Using immunofluorescence microscopy, it was determined that LEF-11 localized to dense regions of infected cell nuclei, consistent with its role as a possible late transcription factor.
苜蓿银纹夜蛾多核衣壳核型多角体病毒（AcMNPV）的lef - 11基因先前被发现对于支持AcMNPV晚期启动子的瞬时表达达到最佳水平是必需的。lef - 11基因不同寻常之处在于它与上游（orf38）和下游（pp31）基因都存在重叠。在本研究中，对LEF - 11的表达和细胞定位进行了检测。在感染后4至36小时（p.i.）检测到lef - 11转录本。1.5 kb的lef - 11 mRNA在lef - 11翻译起始密码子上游196 nt处起始，位于上游的orf38基因内。这个相对较长的5'上游区域编码一个由58个氨基酸组成的潜在小上游开放阅读框（ORF），它与lef - 11 ORF重叠。lef - 11 mRNA的3'末端被定位为与下游pp31基因的mRNA共末端。使用亲和纯化的抗LEF - 11抗体，发现LEF - 11的表达水平在感染后约8至24小时达到最高，尽管直到感染后72小时仍可检测到LEF - 11。通过免疫荧光显微镜检查确定，LEF - 11定位于受感染细胞核的致密区域，这与其作为可能的晚期转录因子的作用一致。
