Mielcarek N, Alonso S, Locht C
INSERM U447, IBL, Institut Pasteur of Lille, 1 Rue du Pr. Calmette, 59019, Lille, France.
Adv Drug Deliv Rev. 2001 Sep 23;51(1-3):55-69. doi: 10.1016/s0169-409x(01)00168-5.
Live recombinant bacteria represent an attractive means to induce both mucosal and systemic immune responses against heterologous antigens. Several models have now been developed and shown to be highly efficient following intranasal immunization. In this review, we describe the two main classes of live recombinant bacteria: generally recognized as safe bacteria and attenuated strains derived from pathogenic bacteria. Among the latter, we have differentiated the bacteria, which do not usually colonize the respiratory tract from those that are especially adapted to respiratory tissues. The strategies of expression of the heterologous antigens, the invasiveness and the immunogenicity of the recombinant bacteria are discussed.
活重组细菌是诱导针对异源抗原的黏膜和全身免疫反应的一种有吸引力的手段。目前已经开发了几种模型，并显示在鼻内免疫后具有高效性。在这篇综述中，我们描述了两类主要的活重组细菌：一般认为安全的细菌和源自病原菌的减毒株。在后者中，我们区分了通常不在呼吸道定植的细菌和特别适应呼吸道组织的细菌。讨论了重组细菌中异源抗原的表达策略、侵袭性和免疫原性。
