人类DNA复制起始因子ORC和MCM与爱泼斯坦-巴尔病毒的oriP相关联。
Human DNA replication initiation factors, ORC and MCM, associate with oriP of Epstein-Barr virus.
作者信息
Chaudhuri B, Xu H, Todorov I, Dutta A, Yates J L
机构信息
Department of Cancer Genetics, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
出版信息
Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10085-9. doi: 10.1073/pnas.181347998. Epub 2001 Aug 21.
Abstract
The 165-kb chromosome of Epstein-Barr virus (EBV) is replicated by cellular enzymes only once per cell cycle in human cells that are latently infected. Here, we report that the human origin recognition complex, ORC, can be detected in association with an EBV replication origin, oriP, in cells by using antibodies against three different subunits of human ORC to precipitate crosslinked chromatin. Mcm2, a subunit of the MCM replication licensing complex, was found to associate with oriP during G(1) and to dissociate from it during S phase. The detection of ORC and Mcm2 at oriP was shown to require the presence of the 120-bp replicator of oriP. Licensing and initiation of replication at oriP of EBV thus seem to be mediated by ORC. This is an example of a virus apparently using ORC and associated factors for the propagation of its genome.
摘要
在潜伏感染的人类细胞中，爱泼斯坦-巴尔病毒（EBV）的165千碱基对染色体仅在每个细胞周期被细胞酶复制一次。在此，我们报告，通过使用针对人类起始识别复合物（ORC）三种不同亚基的抗体沉淀交联染色质，可在细胞中检测到与EBV复制起点oriP相关联的人类ORC。MCM复制许可复合物的一个亚基Mcm2，被发现在G1期与oriP相关联，并在S期与之解离。结果表明，在oriP处检测到ORC和Mcm2需要oriP的120碱基对复制子的存在。因此，EBV oriP处的复制许可和起始似乎由ORC介导。这是一个病毒显然利用ORC及相关因子进行其基因组复制的例子。
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