Crawford P C, Papadi G P, Levy J K, Benson N A, Mergia A, Johnson C M
Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, 32610-0880, USA.
J Infect Dis. 2001 Sep 15;184(6):671-81. doi: 10.1086/323076. Epub 2001 Aug 24.
The purpose of this study was to determine the tissue distribution and antiviral activity of the CD8 lymphocytes that suppress the replication of feline immunodeficiency virus (FIV). Cell-associated FIV load, CD8alpha(+)beta(low) cells, and CD8 cell-mediated suppression of FIV were measured serially in the blood, thymus, and peripheral lymph nodes after neonatal inoculation. Between 6 and 10 weeks, relative numbers of CD8alpha(+)beta(low) cells increased, whereas CD8alpha(+)beta(high) cells declined in the thymus and blood of infected cats. By 12-16 weeks, the lymph nodes were enlarged because of an absolute expansion of all CD8beta subpopulations. The strength of CD8 cell-mediated FIV suppression in vitro, but not CD8alpha(+)beta(low) cell content, was correlated inversely with virus load in the thymus and blood. Thus, after neonatal FIV inoculation, CD8alpha(+)beta(low) cells first occupy the thymus and blood, where strong CD8 cell-mediated antiviral activity is linked to reduced virus load in multiple lymphoid tissues.
本研究的目的是确定抑制猫免疫缺陷病毒（FIV）复制的CD8淋巴细胞的组织分布和抗病毒活性。在新生猫接种后，连续测量血液、胸腺和外周淋巴结中的细胞相关FIV载量、CD8α(+)β(low)细胞以及CD8细胞介导的FIV抑制作用。在6至10周之间，感染猫的胸腺和血液中CD8α(+)β(low)细胞的相对数量增加，而CD8α(+)β(high)细胞数量下降。到12 - 16周时，由于所有CD8β亚群的绝对扩增，淋巴结肿大。体外CD8细胞介导的FIV抑制强度与胸腺和血液中的病毒载量呈负相关，而与CD8α(+)β(low)细胞含量无关。因此，新生猫接种FIV后，CD8α(+)β(low)细胞首先占据胸腺和血液，在这些部位，强大的CD8细胞介导的抗病毒活性与多个淋巴组织中病毒载量的降低有关。
