感染猫免疫缺陷病毒的猫体内CD8α+β-细胞的扩增。

Expansion of CD8alpha+beta- cells in cats infected with feline immunodeficiency virus.

作者信息

Shimojima M, Miyazawa T, Kohmoto M, Ikeda Y, Nishimura Y, Maeda K, Tohya Y, Mikami T

机构信息

Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.

出版信息

J Gen Virol. 1998 Jan;79 ( Pt 1):91-4. doi: 10.1099/0022-1317-79-1-91.

DOI:10.1099/0022-1317-79-1-91

PMID:9460928

Abstract

CD8+ lymphocytes have been subdivided into CD8alphabeta and CD8alpha alpha populations in the peripheral blood lymphocytes (PBL) of humans and in several animal species but have not yet been investigated in cats. Feline immunodeficiency virus (FIV) causes progressive immunological disorders similar to human AIDS. In this study, we analysed CD8+ cells in PBL of FIV-infected or uninfected cats by two-colour flow cytometric analysis. In specific pathogen-free adult cats, feline CD8alpha+beta(high) cells were observed but CD8alpha+beta- cells were not found in significant numbers. On the other hand, not only CD8alpha+beta(high) but also CD8alpha+beta- and CD8alpha+beta(low) cell populations were observed in cats chronically infected with FIV. The expansion of the CD8beta(low) or CD8beta- subpopulations resulted in the apparent differences in CD4/CD8 ratios depending on the anti-CD8 MAb used. These findings suggest a need to reconsider the CD4/CD8 ratio in studies of FIV infection. Furthermore, we found that the CD8alpha+beta- cell population expressed CD5 at a low level.

摘要

在人类及多种动物的外周血淋巴细胞（PBL）中，CD8⁺淋巴细胞已被细分为CD8αβ和CD8αα群体，但尚未在猫身上进行研究。猫免疫缺陷病毒（FIV）会引发类似于人类艾滋病的进行性免疫紊乱。在本研究中，我们通过双色流式细胞术分析了FIV感染或未感染猫的PBL中的CD8⁺细胞。在无特定病原体的成年猫中，观察到了猫CD8α⁺β（高）细胞，但未发现大量的CD8α⁺β⁻细胞。另一方面，在长期感染FIV的猫中，不仅观察到了CD8α⁺β（高）细胞群体，还观察到了CD8α⁺β⁻和CD8α⁺β（低）细胞群体。CD8β（低）或CD8β⁻亚群的扩增导致根据所用抗CD8单克隆抗体的不同，CD4/CD8比值出现明显差异。这些发现表明，在FIV感染研究中需要重新考虑CD4/CD8比值。此外，我们发现CD8α⁺β⁻细胞群体低水平表达CD5。