人眼中视紫红质的闪光光解作用。
Rhodopsin flash photolysis in man.
作者信息
Pugh E N
出版信息
J Physiol. 1975 Jun;248(2):393-412. doi: 10.1113/jphysiol.1975.sp010981.
Abstract
- Human rhodopsin in vivo was flash bleached by a 600 musec xenon flash which could deliver to the retina up to 15 rod-equivalent quanta per rhodopsin molecule, and the fraction bleached measured by fundus reflexion densitometry. 2. The curve relating fraction rhodopsin bleached to intensity of flash saturates at 0.5 to 0.6. Thus, 40-50% of the rhodopsin is left photo-regenerated by the brightest flashes. 3. Three types of densitometry experiments confirm the saturation of the bleaching curve. 4. The kinetic constants required to account for the observed photo-regeneration were somewhat discrepant with in vitro and in situ estimates from infrahuman species. Specifically, (i) the quantum efficiency of the back reaction, metarhodopsin I hv leads to rhodopsin, was inferred to be nearly as high as that of the forward reaction; and (ii) the rate of the metarhodopsin I leads to metarhodopsin II dark reaction was inferred to be less than 500 sec(-1).
摘要
- 人视紫红质在体内通过600微秒的氙闪光进行闪光漂白，该闪光可向视网膜传递高达每视紫红质分子15个视杆等效量子，漂白部分通过眼底反射密度测定法测量。2. 将视紫红质漂白部分与闪光强度相关的曲线在0.5至0.6处达到饱和。因此，最亮的闪光可使40 - 50%的视紫红质留下进行光再生。3. 三种类型的密度测定实验证实了漂白曲线的饱和。4. 解释观察到的光再生所需的动力学常数与来自非人类物种的体外和原位估计有些差异。具体而言，(i) 逆反应（变视紫红质I hv生成视紫红质）的量子效率被推断几乎与正向反应一样高；(ii) 变视紫红质I生成变视紫红质II的暗反应速率被推断小于500秒⁻¹。
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