Tolmacheva T A, Kats L N, Grekova N A
Zh Mikrobiol Epidemiol Immunobiol. 1979 Aug(8):63-7.
On the basis of changes in the biological properties and morphology of Br. abortus culture under the action of penicillin 3 stages of L-transformation in Brucella were determined. The prevalence of first bacilliform and then typical L-cells and rapid reversion hampering the determination of virulence were characteristic of the initial stage (passages 1-4). Typical L-cells with the wrinkled surface, deep depressions and holes as well as a decrease in virulence and slight pathomorphological changes in the organs of the infected animals were characteristics of the intermediate stage (passages 5-10). Typical L-cells and amorphous masses, a further decrease in virulence, pathomorphological changes of toxic character (only after the injection of L-culture in large doses) were characteristic of the late stage (from passage 11 and further on). At all stages of L-transformation Brucella cultures showed a high reproductive capacity, binary division, the formation of elementary bodies by budding both inside and on the surface of L-cells.
根据青霉素作用下流产布鲁氏菌培养物生物学特性和形态学的变化，确定了布鲁氏菌L型转化的3个阶段。初始阶段（第1 - 4代）的特征是先出现杆状菌，然后是典型的L型细胞，且快速回复，这妨碍了毒力的测定。中间阶段（第5 - 10代）的特征是具有皱缩表面、深陷和孔洞的典型L型细胞，毒力降低，以及感染动物器官出现轻微病理形态学变化。后期阶段（从第11代及以后）的特征是典型L型细胞和无定形团块，毒力进一步降低，以及有毒性特征的病理形态学变化（仅在大剂量注射L型培养物后出现）。在L型转化的所有阶段，布鲁氏菌培养物均表现出高繁殖能力、二分裂、在L型细胞内部和表面通过出芽形成原体。
