Hatten B A, Schulze M L, Huang S Y, Sulkin S E
J Bacteriol. 1969 Aug;99(2):611-8. doi: 10.1128/jb.99.2.611-618.1969.
Brucella abortus L-forms were induced by 5.0 or 10.0 mug of penicillin/ml in a broth medium containing 0.3 m sucrose, and in a semisolid medium containing 10% calf serum and 20.0, 40.0, or 60.0 mug of penicillin/ml. After 96 hr of incubation, L-forms of various sizes and shapes were observed. Basic structures of the L-forms were similar whether induced in liquid or semisolid medium. L-forms had two "unit" membranes, each consisting of two outer dense layers separated by a lucent layer. A few large, irregularly shaped organisms in penicillin-treated broth cultures had additional surface material and were referred to as "transitional" forms. In contrast with L-forms, the bacterial cells were fairly uniform in size and shape, were smaller, and had a more complex cell wall structure. Small bodies limited by a "unit" membrane were present within and around numerous L-forms from liquid and semisolid medium cultures. Other internal membranous structures were also seen in some L-forms. Most Brucella L-forms described in this paper reverted to bacteria in the absence of penicillin and were structurally characteristic of unstable L-forms.
用含0.3m蔗糖的肉汤培养基中5.0或10.0μg/ml青霉素，以及含10%小牛血清和20.0、40.0或60.0μg/ml青霉素的半固体培养基诱导流产布鲁氏菌L型。培养96小时后，观察到各种大小和形状的L型。无论在液体还是半固体培养基中诱导，L型的基本结构相似。L型有两个“单位”膜，每个膜由两个由透明层隔开的外部致密层组成。青霉素处理的肉汤培养物中一些大的、形状不规则的生物体有额外的表面物质，被称为“过渡”形式。与L型相比，细菌细胞在大小和形状上相当均匀，较小，且细胞壁结构更复杂。在来自液体和半固体培养基培养物的许多L型内部和周围存在由“单位”膜限制的小体。在一些L型中也可见其他内部膜结构。本文描述的大多数布鲁氏菌L型在无青霉素的情况下可回复为细菌，并且是不稳定L型的结构特征。
