Bateman T A, Dunstan C R, Lacey D L, Ferguson V L, Ayers R A, Simske S J
Department of Aerospace Engineering Sciences, BioServe Space Technologies, University of Colorado, Boulder 80309-0429, USA.
J Orthop Res. 2001 Jul;19(4):518-23. doi: 10.1016/S0736-0266(00)00057-7.
This study examines the ability of osteoprotegerin (OPG) to prevent the local bone resorption caused by sciatic nerve damage. Sixty-five 18-week-old male mice were assigned to one of six groups (n = 10-11/group). A baseline control group was sacrificed on day zero of the 10-day study. The remaining groups were placebo sham operated, placebo nerve crush (Plac NC) operated, 0.1 mg/kg/day OPG + nerve crush (LOW), 0.3 mg/kg/day OPG + nerve crush (MED), and 1.0 mg/kg/day OPG + nerve crush (HI). Nerve crush or sham operations were performed on the right leg. The left leg served as a contralateral control to the nerve crushed (ipsilateral) leg. The difference in mass between the right and left femur and tibia was examined. Additionally, quantitative histomorphometry was performed on the right and left femur and tibia diaphyses. Nerve crush resulted in a significant loss of bone mass in the ipsilateral side compared to the contralateral side. Bone mass for the ipsilateral bones of the Plac NC group were significantly reduced by 3.8% in the femur and 3.5% in the tibia compared to the contralateral limb. The percent diminution was reduced for OPG treated mice compared to the Plac NC group for both the femur and tibia. In the femur, the percent reduction of ipsilateral bone mass was reduced to 1.0% (LOW), 1.3% (MED) and 1.6% (HI) compared to the contralateral limb. In the tibia, loss of bone mass in the ipsilateral limb was reduced to 1.4% (LOW), 1.4% (MED), and 2.4% (HI) compared to the contralateral. OPG also decreased the amount of tibial endocortical resorption compared to the Plac NC group. In summary, OPG mitigated bone loss caused by damage to the sciatic nerve.
本研究考察了骨保护素（OPG）预防坐骨神经损伤所致局部骨吸收的能力。将65只18周龄雄性小鼠分为六组之一（每组n = 10 - 11只）。在为期10天的研究的第0天处死一个基线对照组。其余组分别为假手术安慰剂组、神经挤压安慰剂组（Plac NC）、0.1 mg/kg/天OPG + 神经挤压组（低剂量组）、0.3 mg/kg/天OPG + 神经挤压组（中剂量组）和1.0 mg/kg/天OPG + 神经挤压组（高剂量组）。对右腿进行神经挤压或假手术。左腿作为与神经挤压（同侧）腿相对的对侧对照。检测右、左股骨和胫骨之间的质量差异。此外，对右、左股骨和胫骨骨干进行定量组织形态计量学分析。与对侧相比，神经挤压导致同侧骨量显著减少。与对侧肢体相比，Plac NC组同侧股骨的骨量显著减少3.8%，胫骨减少3.5%。与Plac NC组相比，OPG治疗小鼠的股骨和胫骨骨量减少百分比均降低。在股骨中，同侧骨量减少百分比与对侧肢体相比分别降至1.0%（低剂量组）、1.3%（中剂量组）和1.6%（高剂量组）。在胫骨中，与对侧相比，同侧肢体骨量损失分别降至1.4%（低剂量组）、1.4%（中剂量组）和2.4%（高剂量组）。与Plac NC组相比，OPG还减少了胫骨内皮质吸收量。总之，OPG减轻了坐骨神经损伤所致的骨质流失。
