Hilhorst M J, Somsen G W, de Jong G J
Department of Pharmaceutical Analysis, University of Groningen, The Netherlands.
Electrophoresis. 2001 Aug;22(12):2542-64. doi: 10.1002/1522-2683(200107)22:12<2542::AID-ELPS2542>3.0.CO;2-3.
Capillary electrokinetic separation techniques offer high efficiency and peak capacity, and can be very useful for the analysis of samples containing a large variety of (unknown) compounds. Such samples are frequently met in impurity profiling of drugs (detection of potential impurities in a pharmaceutical substance or product) and in general sample profiling (determination of differences or similarities between samples). In this paper, the potential, merits, and limitations of electrokinetic separation techniques for profiling purposes are evaluated using examples from literature. A distinction is made between impurity profiling, forensic profiling and profiling of natural products, and the application of capillary zone electrophoresis, micellar electrokinetic chromatography, and capillary electrochromatography in these fields is discussed. Attention is devoted to important aspects such as selectivity, resolution enhancement, applicability, detection, and compound confirmation and quantification. The specific properties of the various electrokinetic techniques are discussed and compared with more conventional techniques as liquid chromatography.
毛细管电动分离技术具有高效性和高柱容量，对于分析含有多种（未知）化合物的样品非常有用。这类样品在药物杂质剖析（检测药物物质或产品中的潜在杂质）和一般样品剖析（确定样品之间的差异或相似性）中经常遇到。本文通过文献实例评估了用于剖析目的的电动分离技术的潜力、优点和局限性。区分了杂质剖析、法医剖析和天然产物剖析，并讨论了毛细管区带电泳、胶束电动色谱和毛细管电色谱在这些领域的应用。重点关注了选择性、分辨率提高、适用性、检测以及化合物确认和定量等重要方面。讨论了各种电动技术的具体特性，并与更传统的技术如液相色谱进行了比较。
