Tagliaro F, Turrina S, Pisi P, Smith F P, Marigo M
Institute of Forensic Medicine, University of Verona, Italy.
J Chromatogr B Biomed Sci Appl. 1998 Aug 21;713(1):27-49. doi: 10.1016/s0378-4347(97)00485-4.
The application of capillary electrophoresis (CE) methods in forensic toxicology for the determination of illicit and/or misused drugs in biological samples is reviewed in the present paper. Sample pretreatments and direct injection modes used in CE for analysis of drugs in biological fluids are briefly described. Besides, applications of separation methods based on capillary zone electrophoresis or micellar electrokinetic chromatography with UV absorbance detection to (i) analysis of drugs of abuse, (ii) analysis of other drugs and toxicants of potential forensic interest and (iii) for metabolism studies are reviewed. Also, alternative CE methods are briefly discussed, including capillary isotachophoresis and separation on mixed polymer networks. High sensitivity detection methods used for forensic drug analysis in biological samples are then presented, particularly those based on laser induced fluorescence. A glimpse of the first examples of application of CE-mass spectrometry in forensic toxicology is finally given.
本文综述了毛细管电泳（CE）方法在法医毒理学中用于测定生物样品中非法和/或滥用药物的应用。简要描述了CE用于分析生物流体中药物的样品预处理和直接进样模式。此外，还综述了基于毛细管区带电泳或胶束电动色谱并结合紫外吸收检测的分离方法在以下方面的应用：（i）滥用药物分析；（ii）其他具有潜在法医意义的药物和毒物分析；（iii）代谢研究。此外，还简要讨论了替代CE方法，包括毛细管等速电泳和在混合聚合物网络上的分离。随后介绍了用于生物样品中法医药物分析的高灵敏度检测方法，特别是基于激光诱导荧光的方法。最后简要介绍了CE-质谱在法医毒理学中的首批应用实例。
