D-核酮糖-1,5-二磷酸羧化酶I型晶体的结构
The structure of form I crystals of D-ribulose-1,5-diphosphate carboxylase.
作者信息
Baker T S, Eisenberg D, Eiserling F A, Weissman L
出版信息
J Mol Biol. 1975 Feb 5;91(4):391-9. doi: 10.1016/0022-2836(75)90267-3.
Abstract
Single crystals of d-ribulose-1,5-diphosphate carboxylase from tobacco leaves, (variety Turkish Samsun), have been examined by X-ray diffraction, electron microscopy, and optical diffraction. Twelve molecules are loosely packed into a body-centered cubic unit cell, space group 1432 with cell dimension = 383 Å. The asymmetric unit is one quarter of a molecule, and the minimum molecular symmetry is 222. This symmetry when combined with estimates of the two subunit masses and stoichiometry is compatible with a molecular structure of the composition LS (L is large subunit, S is small). If all bonds between large and small subunits are equivalent, the true molecular symmetry is 422; this symmetry is consistent with molecular images in micrographs.
摘要
已通过X射线衍射、电子显微镜和光学衍射对来自烟草叶片（品种为土耳其萨姆松）的1,5-二磷酸核酮糖羧化酶单晶进行了研究。十二个分子松散地堆积在一个体心立方晶胞中，空间群为1432，晶胞尺寸a = 383 Å。不对称单元是一个分子的四分之一，最小分子对称性为222。这种对称性与两个亚基质量和化学计量的估计值相结合，与组成LS（L是大亚基，S是小亚基）的分子结构相符。如果大亚基和小亚基之间的所有键都是等效的，那么真正的分子对称性是422；这种对称性与显微照片中的分子图像一致。
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