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A method is described for the extraction and purification of Fraction I protein from Avena sativa L. leaves. 2. The protein possesses ribulose diphosphate carboxylase activity. Chromatography on gels of Sephadex G-200 separates phosphoribulokinase and ribose phosphate isomerase from the carboxylase. 3. The S°20w was calculated to be 18.2, the Stokes radius (determined by gel filtration on a cabibrated column) 74 Å, the molecular weight 5.7×10(5), and the frictional ratio 1.35. 4. An amino acid analysis is presented. 5. Electron microscope observations of negatively-stained Avena Fraction I protein molecules are compatible with the suggestion that they consist of 24 protomers disposed on the surface of an octahedral shell with 4:3:2 symmetry, and of diameter approximately 105 Å.

描述了从燕麦叶片中提取和纯化 Fraction I 蛋白的方法。
该蛋白具有核酮糖二磷酸羧化酶活性。Sephadex G-200 凝胶层析将磷酸核酮激酶和核糖磷酸异构酶与羧化酶分离。
S°20w 计算为 18.2，Stokes 半径（通过在 cabibrated 柱上的凝胶过滤确定）为 74 Å，分子量为 5.7×10(5)，摩擦比为 1.35。
提供了氨基酸分析。
negatively-stained Avena Fraction I 蛋白分子的电子显微镜观察结果与以下假设一致，即它们由 24 个亚基组成，排列在具有 4:3:2 对称性的八面体壳的表面上，直径约为 105 Å。

A method is described for the extraction and purification of Fraction I protein from Avena sativa L. leaves. 2. The protein possesses ribulose diphosphate carboxylase activity. Chromatography on gels of Sephadex G-200 separates phosphoribulokinase and ribose phosphate isomerase from the carboxylase. 3. The S°20w was calculated to be 18.2, the Stokes radius (determined by gel filtration on a cabibrated column) 74 Å, the molecular weight 5.7×10(5), and the frictional ratio 1.35. 4. An amino acid analysis is presented. 5. Electron microscope observations of negatively-stained Avena Fraction I protein molecules are compatible with the suggestion that they consist of 24 protomers disposed on the surface of an octahedral shell with 4:3:2 symmetry, and of diameter approximately 105 Å.

描述了从燕麦叶片中提取和纯化 Fraction I 蛋白的方法。
该蛋白具有核酮糖二磷酸羧化酶活性。Sephadex G-200 凝胶层析将磷酸核酮激酶和核糖磷酸异构酶与羧化酶分离。
S°20w 计算为 18.2，Stokes 半径（通过在 cabibrated 柱上的凝胶过滤确定）为 74 Å，分子量为 5.7×10(5)，摩擦比为 1.35。
提供了氨基酸分析。
negatively-stained Avena Fraction I 蛋白分子的电子显微镜观察结果与以下假设一致，即它们由 24 个亚基组成，排列在具有 4:3:2 对称性的八面体壳的表面上，直径约为 105 Å。

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