Green C L, Frommer M
Fruit Fly Research Centre, School of Biological Sciences, University of Sydney, New South Wales 2006, Australia.
Insect Mol Biol. 2001 Aug;10(4):371-86. doi: 10.1046/j.0962-1075.2001.00275.x.
Representatives of five distinct types of transposable elements of the mariner family were detected in the genomes of the Queensland fruit fly Bactrocera tryoni and its sibling species Bactrocera neohumeralis by phylogenetic analysis of transposase gene fragments. Three mariner types were also found in an additional tephritid, Bactrocera jarvisi. Using genomic library screening and inverse PCR, full-length elements representing the mellifera subfamily (B. tryoni.mar1) and the irritans subfamily (B. tryoni.mar2) were isolated from the B. tryoni genome. Nucleotide consensus sequences for each type were derived from multiple defective copies. Predicted transposase sequences share approximately 23% amino acid identity. B. tryoni.mar1 elements have an estimated copy number of about 900 in the B. tryoni genome, whereas B. tryoni.mar2 element types appear to be present in low copy number.
通过对转座酶基因片段进行系统发育分析,在昆士兰果蝇Bactrocera tryoni及其近缘种Bactrocera neohumeralis的基因组中检测到了水手家族五种不同类型的转座元件。在另外一种实蝇Bactrocera jarvisi中也发现了三种水手类型。利用基因组文库筛选和反向PCR技术,从B. tryoni基因组中分离出了代表蜜蜂亚科(B. tryoni.mar1)和刺激亚科(B. tryoni.mar2)的全长元件。每种类型的核苷酸共有序列来自多个缺陷拷贝。预测的转座酶序列氨基酸同一性约为23%。B. tryoni.mar1元件在B. tryoni基因组中的估计拷贝数约为900个,而B. tryoni.mar2元件类型似乎以低拷贝数存在。