Raphael K A, Shearman D C A, Streamer K, Morrow J L, Handler A M, Frommer M
Fruit Fly Research Laboratories, School of Biological Sciences, University of Sydney, Sydney, NSW, 2006, Australia.
Genetica. 2011 Jan;139(1):91-7. doi: 10.1007/s10709-010-9500-x. Epub 2010 Sep 22.
We report the heritable germ-line transformation of the Queensland fruit fly, Bactrocera tryoni, using a piggyBac vector marked with either the fluorescent protein DsRed or EGFP. A transformation frequency of 5-10% was obtained. Inheritance of the transgenes has remained stable over more than 15 generations despite the presence of endogenous piggyBac sequences in the B. tryoni genome. The sequence of insertion sites shows the usual canonical pattern of piggyBac integraton into TTAA target sites. An investigation of endogenous piggyBac elements in the B. tryoni genome reveals the presence of sequences almost identical to those reported recently for the B. dorsalis complex of fruit flies and two noctuid moths, suggesting a common origin of piggyBac sequences in these species. The availability of transformation protocols for B. tryoni has the potential to deliver improvements in the performance of the Sterile Insect Technique for this pest species.
我们报告了使用标记有荧光蛋白DsRed或EGFP的piggyBac载体对昆士兰果蝇（Bactrocera tryoni）进行可遗传的种系转化。获得了5%-10%的转化频率。尽管昆士兰果蝇基因组中存在内源性piggyBac序列，但转基因的遗传在超过15代中一直保持稳定。插入位点的序列显示了piggyBac整合到TTAA靶位点的常见典型模式。对昆士兰果蝇基因组中的内源性piggyBac元件的研究揭示了存在与最近报道的果蝇背侧复合体和两种夜蛾几乎相同的序列，这表明这些物种中piggyBac序列有共同的起源。昆士兰果蝇转化方案的可用性有可能改善这种害虫的不育昆虫技术性能。
