Redondo-Nevado J, Moyano E, Medina-Escobar N, Caballero J L, Muñoz-Blanco J
Departamento de Bioquímica y Biología Molecular, Edificio C-6, Campus Universitario de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain.
J Exp Bot. 2001 Sep;52(362):1941-5. doi: 10.1093/jexbot/52.362.1941.
A fruit-specific and developmentally regulated polygalacturonase gene (spG gene) from strawberry (Fragaria x ananassa cv. Chandler) has been cloned and characterized at a molecular and physiological level. Comparison analysis of the corresponding deduced sPG protein have shown that this strawberry gene is similar to Clade A endopolygalacturonase genes. Moreover, the spatio-temporal and hormonal gene expression pattern suggests a close relationship between the expression of this gene and the onset of the strawberry fruit ripening process and agrees with that of the production of oligosaccharins which have already been described as active molecules involved in fruit ripening. The results are discussed in terms of a putative role of this enzyme in the release of oligosaccharins from the strawberry fruit cell wall.
从草莓（凤梨草莓品种钱德勒）中克隆出了一个果实特异性且受发育调控的多聚半乳糖醛酸酶基因（spG基因），并在分子和生理水平上对其进行了表征。对相应推导的sPG蛋白的比较分析表明，该草莓基因与A类内切多聚半乳糖醛酸酶基因相似。此外，时空和激素基因表达模式表明该基因的表达与草莓果实成熟过程的开始密切相关，并且与已被描述为参与果实成熟的活性分子的寡糖素的产生一致。从该酶在从草莓果实细胞壁释放寡糖素中的假定作用方面对结果进行了讨论。
