Mai J K, Post S
Acta Histochem. 1979;64(2):157-63.
It is often desirable to delineate the areas of autoradiograms where accumulations of silver grains are observed in order to establish topographic landmarks. This is prevented by either bright field or dark field illuminatiated photographs, where the topography of the silver grains is neglected despite the high resolution obtainable by autoradiography, but may be done by taking advantage of a modification of dark field illumination thus generating an additional bright field effect. This modification also enables to correlate selective histochemical reaction sites with the area of silver grain accumulation.
为了建立地形学标志，通常需要描绘出自显影图中观察到银颗粒聚集的区域。明场或暗场照明照片会妨碍这一点，尽管放射自显影可获得高分辨率，但在这些照片中银颗粒的地形被忽略了，但可以通过利用暗场照明的一种改进方法来实现，从而产生额外的明场效果。这种改进还能够将选择性组织化学反应位点与银颗粒聚集区域相关联。
