Gatzke H D, Jennissen J J
Acta Histochem. 1976;56(2):325-34.
KRAUS and GIESE - as proposed by O'CALLAGHAN, STEVENS and WOOD-- could prevent latent image fading throughout exposure with 89Sr/90 Y by addition of 10 mg KBr and 50 g Glucose per liter of water for flotation of stripping films. This method is not useful for the autoradiography of biological material labelled with tritium. Autoradiograms of rat brain slices were investigated after application 3H-leucine, 3H-methionine, 3H-asparagic-acid and 3H-histidine. By adding the above mentioned salts there was an increase of artifacts and a decrease in reproducibility.
克劳斯和吉泽——正如奥卡拉汉、史蒂文斯和伍德所提议的那样——通过在每升用于剥离膜漂浮的水中添加10毫克溴化钾和50克葡萄糖，能够防止在使用89Sr/90Y进行曝光期间潜影褪色。该方法对于用氚标记的生物材料的放射自显影并不适用。在用3H-亮氨酸、3H-蛋氨酸、3H-天冬氨酸和3H-组氨酸处理后，对大鼠脑切片的放射自显影片进行了研究。通过添加上述盐类，伪影增加且再现性降低。
