通过毛细管电泳分离并直接紫外检测与吡啶 - 2 - 羧酸络合的镧系元素。

Separation and direct UV detection of lanthanides complexed with pyridine-2-carboxylic acid by capillary electrophoresis.

作者信息

Oztekin N, Erim F B

机构信息

Department of Chemistry, Technical University of Istanbul, Turkey.

出版信息

J Chromatogr A. 2001 Jul 27;924(1-2):541-6. doi: 10.1016/s0021-9673(01)01045-7.

DOI:10.1016/s0021-9673(01)01045-7

PMID:11521908

Abstract

Separation and detection of lanthanides by capillary zone electrophoresis in the presence of pyridine-2-carboxylic acid (picolinic acid) as UV-absorbing complexing agent were investigated. The resolution of partially complexed positively charged complexes is improved by using two buffer ligands competing with picolinic acid for metal ions. When hydroxyisobutyric acid (HIBA) and formic acid are used together as competing ligands, this provides complete separation of all 14 lanthanides with good peak shapes. An on-column separation of 14 lanthanides was achieved in only 9 min using 0.8 mmol/l picolinic acid, 10 mmol/l HIBA and 25 mmol/l formic acid at pH 4.7. Determination of lanthanide complexes was performed by direct detection at 210 nm. Detection limits (signal-to-noise ratio=3) are ca. 0.53-0.96 microg/ml.

摘要

研究了在吡啶 - 2 - 羧酸（皮考林酸）作为紫外吸收络合剂存在下，通过毛细管区带电泳分离和检测镧系元素。通过使用两种与皮考林酸竞争金属离子的缓冲配体，部分络合的带正电络合物的分离度得到提高。当羟基异丁酸（HIBA）和甲酸一起用作竞争配体时，可实现所有14种镧系元素的完全分离，且峰形良好。在pH 4.7条件下，使用0.8 mmol/L皮考林酸、10 mmol/L HIBA和25 mmol/L甲酸，仅9分钟即可实现14种镧系元素的柱上分离。通过在210 nm处直接检测来测定镧系元素络合物。检测限（信噪比 = 3）约为0.53 - 0.96 μg/ml。