使用2,6-吡啶二甲酸对金属离子进行柱上络合，并通过直接紫外检测的毛细管电泳分离其阴离子络合物。

On-column complexation of metal ions using 2,6-pyridinedicarboxylic acid and separation of their anionic complexes by capillary electrophoresis with direct UV detection.

作者信息

Chen Zuliang, Naidu Ravendra

机构信息

CSIRO Land and Water, Adelaide Laboratory, Glen Osmond, SA, Australia.

出版信息

J Chromatogr A. 2002 Aug 9;966(1-2):245-51. doi: 10.1016/s0021-9673(02)00741-0.

DOI:10.1016/s0021-9673(02)00741-0

PMID:12214701

Abstract

On-column complexation of metal ions with 2,6-pyridinedicarboxylate (2,6-PDC) to form anionic complexes enabled their separation by capillary zone electrophoresis with direct UV detection at 214 nm. Nine metal ions, Cu2+, Zn2+, Ni2+, Cd2+ Mn2+, Pb2+, Fe3+, Al3+ and Ca2+, were determined in less than 7 min using 10 mM 2.6-PDC solution containing 0.75 mM tetradecyltrimethylammonium bromide at pH 4.0. Satisfactory working ranges (20-300 microM), detection limits (3-10 microM) and good repeatability of the peak areas (RSD 2.1-4.2%, n=5) were obtained using hydrodynamic injection (30 s). The proposed method was used successfully for the determination of Mn2+, Fe3+, Al3+ and Ca2+ in groundwaters.

摘要

金属离子与2,6 - 吡啶二甲酸（2,6 - PDC）在柱上形成阴离子配合物，使得它们能够通过毛细管区带电泳在214 nm处直接紫外检测进行分离。使用含有0.75 mM溴化十四烷基三甲基铵的10 mM 2,6 - PDC溶液在pH 4.0条件下，在不到7分钟内测定了9种金属离子，即Cu2 +、Zn2 +、Ni2 +、Cd2 +、Mn2 +、Pb2 +、Fe3 +、Al3 +和Ca2 +。采用流体动力学进样（30 s）获得了令人满意的工作范围（20 - 300 μM）、检测限（3 - 10 μM）以及良好的峰面积重复性（RSD 2.1 - 4.2%，n = 5）。所提出的方法成功用于测定地下水中的Mn2 +、Fe3 +、Al3 +和Ca2 +。