Gao F, Trask S A, Hui H, Mamaeva O, Chen Y, Theodore T S, Foley B T, Korber B T, Shaw G M, Hahn B H
Department of Medicine, University of Alabama at Birmingham, 35294, USA.
AIDS Res Hum Retroviruses. 2001 Aug 10;17(12):1217-22. doi: 10.1089/088922201316912826.
Numerous complete human immunodeficiency virus type 1 (HIV-1) genomes have been characterized for contemporary viruses, but few isolates obtained early in the HIV-1 epidemic have been studied. In this article, we describe the molecular characterization of an HIV-1 isolate (83CD003) that was obtained from an AIDS patient in Kinshasa, Democratic Republic of Congo (DRC) in 1983. The complete 83CD003 genome was sequenced in its entirety and found to encode uninterrupted open reading frames for all viral genes. Phylogenetic analysis revealed 83CD003 was a member of the major (M) group of HIV -1, but did not group with any of the known subtypes. Rather, it formed an independent lineage in all regions of its genome that was roughly equidistant from representatives of all other subtypes. Similarly, 83CD003 also did not cluster with any of several unclassified group M sequences that have been reported more recently to circulate in the DRC, suggesting that it may represent an early group M lineage thai is either rare or has gone extinct. The molecular clone of 83CD003 yielded an infectious virus after transfection into mammalian cells and its biological properties can be further studied.
许多完整的人类免疫缺陷病毒1型（HIV-1）基因组已针对当代病毒进行了特征分析，但对HIV-1流行早期获得的分离株研究较少。在本文中，我们描述了1983年从刚果民主共和国金沙萨一名艾滋病患者身上获得的一株HIV-1分离株（83CD003）的分子特征。对完整的83CD003基因组进行了全序列测序，发现其为所有病毒基因编码不间断的开放阅读框。系统发育分析表明，83CD003是HIV-1主要（M）组的成员，但不与任何已知亚型聚类。相反，它在其基因组的所有区域形成了一个独立的谱系，与所有其他亚型的代表大致等距。同样，83CD003也未与最近报道在刚果民主共和国流行的几个未分类的M组序列中的任何一个聚类，这表明它可能代表一个早期的M组谱系，该谱系要么罕见，要么已经灭绝。83CD003的分子克隆在转染到哺乳动物细胞后产生了一种传染性病毒，其生物学特性可进一步研究。
