Yang C, Dash B, Hanna S L, Frances H S, Nzilambi N, Colebunders R C, St Louis M, Quinn T C, Folks T M, Lal R B
HIV Immunology and Diagnostics Branch and HIV and Retrovirology Branch, Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
AIDS Res Hum Retroviruses. 2001 Mar 1;17(4):361-5. doi: 10.1089/08892220150503726.
We have investigated the genetic diversity and potential mosaic genomes of HIV-1 during the early part of the HIV-1 epidemic among commercial sex workers (CSWs) in Kinshasa, Democratic Republic of Congo (formerly Zaire). Serologic analysis revealed that 27 (28.7%) of the 94 specimens were seropositive by both peptide and whole-virus lysate EIAs and that 24 were positive by molecular screening assays, using generic primers that can detect all known groups of HIV-1. Phylogenetic analyses of the gag(p24), C2V3, and gp41 regions of these 24 specimens showed that all were group M; none of them had any evidence of group O, N, or SIVcpz-like sequences. On the basis of env sequence analysis, the 24 group M specimens were classified as subtypes G (37.5%), A (21%), F1 (12.5%), CRF01_AE (8%), D (4%), and H (4%); 3 (12.5%) were unclassifiable (U). Similar analysis of the gag(p24) region revealed that the majority of infections were subtype A; however, one-third of the specimens were subtype G. Parallel analysis of gag(p24) and env regions revealed discordant subtypes in many specimens that may reflect possible dual and/or recombinant viruses. These data suggest a predominance of subtype G (both pure G and recombinant CRF02_AG) during the early part of the epidemic in Kinshasa. Infections with group N or SIVcpz-like viruses were not present among these CSWs in Kinshasa.
我们对刚果民主共和国（原扎伊尔）金沙萨商业性工作者中HIV-1流行早期阶段的HIV-1基因多样性和潜在镶嵌基因组进行了调查。血清学分析显示，94份标本中有27份（28.7%）通过肽和全病毒裂解物酶免疫测定法均呈血清阳性，24份通过分子筛查测定法呈阳性，该方法使用的通用引物可检测所有已知的HIV-1组。对这24份标本的gag（p24）、C2V3和gp41区域进行系统发育分析表明，所有标本均为M组；没有任何证据表明存在O组、N组或SIVcpz样序列。根据env序列分析，24份M组标本被分类为G亚型（37.5%）、A亚型（21%）、F1亚型（12.5%）、CRF01_AE亚型（8%）、D亚型（4%）和H亚型（4%）；3份（12.5%）无法分类（U）。对gag（p24）区域的类似分析表明，大多数感染为A亚型；然而，三分之一的标本为G亚型。对gag（p24）和env区域的平行分析表明，许多标本中存在不一致的亚型，这可能反映了可能的双重和/或重组病毒。这些数据表明，在金沙萨流行早期，G亚型（包括纯G亚型和重组CRF02_AG亚型）占主导地位。在金沙萨的这些商业性工作者中不存在N组或SIVcpz样病毒感染。
