Tang W, Seino K, Ito M, Konishi T, Senda H, Makuuchi M, Kojima N, Mizuochi T
Department of Applied Biochemistry, Tokai University, Hiratsuka, Kanagawa, Japan.
FEBS Lett. 2001 Aug 24;504(1-2):31-5. doi: 10.1016/s0014-5793(01)02759-4.
Direct adhesion of Helicobacter pylori to immobilized glycosphingolipids (GSLs) was compared to that of their corresponding oligosaccharide-conjugated neoglycoconjugates in order to clarify the roles of the carbohydrate and lipid portions of GSLs in H. pylori adhesion. These bacteria were found to adhere to sulfatide, GM3, GalCer and LacCer, but not to ceramide, sphingomyelin, or polyacrylamides conjugated with beta-galactose, lactose, 3'-sialyllactose or 3'-sulfo-beta-galactose. Furthermore, neoglycolipids or bovine serum albumin derivatives with corresponding oligosaccharides were unable to serve as the ligands. H. pylori adhesion to GalCer with alpha-hydroxyl fatty acid was much stronger than GalCer with the non-hydroxyl fatty acid. These results suggest that H. pylori recognize the conformation of GSL with alpha-hydroxyl fatty acid on solid phase.
为阐明鞘糖脂（GSLs）的碳水化合物和脂质部分在幽门螺杆菌黏附中的作用，将幽门螺杆菌与固定化鞘糖脂的直接黏附情况与其相应的寡糖共轭新糖缀合物进行了比较。发现这些细菌能黏附于硫苷脂、GM3、半乳糖神经酰胺和乳糖神经酰胺，但不能黏附于神经酰胺、鞘磷脂或与β-半乳糖、乳糖、3'-唾液酸乳糖或3'-磺基-β-半乳糖共轭的聚丙烯酰胺。此外，具有相应寡糖的新糖脂或牛血清白蛋白衍生物不能作为配体。幽门螺杆菌对含α-羟基脂肪酸的半乳糖神经酰胺的黏附力比对含非羟基脂肪酸的半乳糖神经酰胺的黏附力强得多。这些结果表明，幽门螺杆菌能识别固相上含α-羟基脂肪酸的鞘糖脂的构象。
