Costa Natália R, Mendes Nuno, Marcos Nuno T, Reis Celso A, Caffrey Thomas, Hollingsworth Michael A, Santos-Silva Filipe
Institute of Molecular Pathology and Immunology, University of Porto, Porto 4200-465, Portugal.
World J Gastroenterol. 2008 Mar 7;14(9):1411-4. doi: 10.3748/wjg.14.1411.
To evaluate the influence of MUC1 mucin variable number of tandem repeats (VNTR) variability on H pylori adhesion to gastric cells.
Enzyme linked immunosorbent assay (ELISA)-based adhesion assays were performed to measure the adhesion of different H pylori strains (HP26695 and HPTx30a) to gastric carcinoma cell lines (GP202 and MKN45) and GP202 clones expressing recombinant MUC1 with different VNTR lengths.
Evaluation of adhesion results shows that H pylori pathogenic strain HP26695 has a significantly higher (P<0.05) adhesion to all the cell lines and clones tested, when compared to the non-pathogenic strain HPTx30a. Bacteria showed a significantly higher (P<0.05) adhesion to the GP202 cell line, when compared to the MKN45 cell line. Furthermore, both strains showed a significantly higher (P<0.05) adhesion to GP202 clones with larger MUC1 VNTR domains.
This work shows that MUC1 mucin variability conditions H pylori binding to gastric cells. The extent of bacterial adhesion depends on the size of the MUC1 VNTR domain. The adhesion is further dependent on bacterial pathogenicity and the gastric cell line. MUC1 mucin variability may contribute to determine H pylori colonization of the gastric mucosa.
评估黏蛋白1（MUC1）可变数目串联重复序列（VNTR）变异对幽门螺杆菌黏附胃细胞的影响。
采用基于酶联免疫吸附测定（ELISA）的黏附试验，检测不同幽门螺杆菌菌株（HP26695和HPTx30a）对胃癌细胞系（GP202和MKN45）以及表达不同VNTR长度重组MUC1的GP202克隆的黏附情况。
黏附结果评估显示，与非致病菌株HPTx30a相比，幽门螺杆菌致病菌株HP26695对所有测试的细胞系和克隆的黏附显著更高（P<0.05）。与MKN45细胞系相比，细菌对GP202细胞系的黏附显著更高（P<0.05）。此外，两种菌株对具有较大MUC1 VNTR结构域的GP202克隆的黏附均显著更高（P<0.05）。
本研究表明，MUC1黏蛋白变异影响幽门螺杆菌与胃细胞的结合。细菌黏附程度取决于MUC1 VNTR结构域的大小。黏附还取决于细菌致病性和胃细胞系。MUC1黏蛋白变异可能有助于确定幽门螺杆菌在胃黏膜的定植。
