Yamamoto J, Ohnishi K, Kira J, Konishi N, Yamotori K
Jpn Heart J. 1975 Jul;16(4):446-53. doi: 10.1536/ihj.16.446.
Kidney extract from rats which were adrenalectomized and given tap water was dialyzed, salted out, ultrafiltrated or heated. A given dose of each extract or fractionized material was administered to uninephrectomized rats subcutaneously every 12 hours for 10 days. The relationship between renin content of each sample and final blood pressure level following repeated injections as an index of its hypertension-inducing potency was analyzed. There was no apparent discrepancy between the two of each sample. No evidence was obtained for the existence of other renal substance than renin which might be implicated in producing hypertension.
将摘除肾上腺并给予自来水的大鼠的肾提取物进行透析、盐析、超滤或加热。将每种提取物或分级分离物质的给定剂量每12小时皮下注射给单侧肾切除的大鼠，持续10天。分析每个样品的肾素含量与重复注射后最终血压水平之间的关系，以此作为其诱导高血压效力的指标。每个样品的两者之间没有明显差异。没有获得证据表明除肾素外还存在其他可能与高血压产生有关的肾脏物质。
