Yamamoto J, Kira J, Matsunaga M, Ogino K, Kawai C
Jpn Circ J. 1976 Jan;40(1):21-8. doi: 10.1253/jcj.40.21.
The relationship between hypertension-inducing potency and renin content of kidney extract was analyzed in rats using Sephadex G-100, CM-Sephadex C-50, DEAE-Cellulose or Concanavalin A-Sepharose column chromatography. Hypertension-inducing potency of each fraction obtained was evaluated in the basis of the final blood pressure level attained by repeated injections into the test animals for 10 days. Hypertension-inducing potency was found mainly in the renin-contaning fractions. And it seems reasonable to conclude that renin is implicated in the pathogenesis of hypertension produced by kidney extract. However, there were significant discrepancies between hypertension-inducing potency and renin content of subdivided fractions of these renin-containing eluates. Possible explantations for the discrepancies disclosed--including the possibility of the involvement of an unknown substance(s)--have been discussed. In addition, it was suggested from the results of Concanavalin A-Sepharose chromatography that rat renal renin has a glycoprotein nature.
使用葡聚糖凝胶G - 100、羧甲基葡聚糖凝胶C - 50、二乙氨基乙基纤维素或伴刀豆球蛋白A - 琼脂糖柱色谱法，分析了大鼠肾提取物的致高血压效力与肾素含量之间的关系。根据对试验动物连续注射10天所达到的最终血压水平，评估所得各馏分的致高血压效力。致高血压效力主要存在于含肾素的馏分中。由此得出肾素与肾提取物所致高血压的发病机制有关这一结论似乎是合理的。然而，这些含肾素洗脱液的细分馏分的致高血压效力与肾素含量之间存在显著差异。已对所发现差异的可能解释——包括未知物质参与的可能性——进行了讨论。此外，伴刀豆球蛋白A - 琼脂糖色谱分析结果表明大鼠肾素具有糖蛋白性质。
