Mitchell J H, Duthie S J, Collins A R
Division of Cellular Integrity, Rowett Research Institute, Bucksburn, Aberdeen, UK.
Nutr Cancer. 2000;38(2):223-8. doi: 10.1207/S15327914NC382_12.
Prostate cancer is one of the most common male cancers in Western countries, yet the incidence of this fatal disease remains low in Asian populations. Environmental factors such as diet play an important role in hormone-dependent cancer etiology, and a high phytoestrogen intake may be one factor contributing to the low prostate cancer mortality in Eastern populations. In this study, we investigated the effects of the phytoestrogens genistein, daidzein, coumestrol, and equol on cell growth and DNA damage (strand breakage) in two human prostate tumor cell lines: androgen receptor-positive LNCaP and androgen receptor-negative PC-3. Each compound caused growth inhibition at physiologically relevant concentrations (<10 microM). Genistein induced DNA damage in both cell lines at <10 microM. Daidzein inhibited cell growth at 10-100 microM yet had no effect on DNA damage at up to 500 microM. Thus, despite their structural similarities, different phytoestrogens inhibit prostate tumor cell growth by independent mechanisms.
前列腺癌是西方国家最常见的男性癌症之一，但在亚洲人群中，这种致命疾病的发病率仍然较低。饮食等环境因素在激素依赖性癌症病因中起着重要作用，高植物雌激素摄入量可能是导致东方人群前列腺癌死亡率较低的一个因素。在本研究中，我们研究了染料木黄酮、大豆苷元、香豆雌酚和雌马酚这几种植物雌激素对两种人前列腺肿瘤细胞系（雄激素受体阳性的LNCaP和雄激素受体阴性的PC-3）细胞生长和DNA损伤（链断裂）的影响。每种化合物在生理相关浓度（<10 microM）下均引起生长抑制。染料木黄酮在<10 microM时在两种细胞系中均诱导DNA损伤。大豆苷元在10 - 100 microM时抑制细胞生长，但在高达500 microM时对DNA损伤无影响。因此，尽管它们结构相似，但不同的植物雌激素通过独立的机制抑制前列腺肿瘤细胞生长。
