瘤胃细菌琥珀酸丝状杆菌S85中钠梯度维持的体内23Na核磁共振研究。
In vivo 23Na nuclear magnetic resonance study of maintenance of a sodium gradient in the ruminal bacterium Fibrobacter succinogenes S85.
作者信息
Schwaab V, Matheron C, Delort A M, Gaudet G, Forano E
机构信息
Laboratoire de Synthèse, Electrosynthèse et Etude de Systèmes à Intérêt Biologique, UMR 6504, Université Blaise Pascal-CNRS, 63177 Aubière cedex, France.
出版信息
Appl Environ Microbiol. 2001 Sep;67(9):4390-2. doi: 10.1128/AEM.67.9.4390-4392.2001.
Sodium gradients (DeltapNa) were measured in resting cells of Fibrobacter succinogenes by in vivo 23Na nuclear magnetic resonance using Tm(DOTP)5- [thulium(III) 1,4,7,10-tetraazacyclododecane-N',N",N"'-tetramethylenephosphonate] as the shift reagent. This bacterium was able to maintain a DeltapNa of -55 to -40 mV for extracellular sodium concentrations ranging from 30 to 200 mM. Depletion of Na+ ions during the washing steps led to irreversible damage (modification of glucose metabolism and inability to maintain a sodium gradient).
使用Tm(DOTP)5- [铥(III) 1,4,7,10-四氮杂环十二烷-N',N",N"'-四亚甲基膦酸酯]作为位移试剂，通过体内23Na核磁共振在琥珀酸纤维杆菌的静息细胞中测量钠梯度（ΔpNa）。对于细胞外钠浓度在30至200 mM范围内的情况，这种细菌能够维持-55至-40 mV的ΔpNa。洗涤步骤中Na+离子的耗尽导致不可逆损伤（葡萄糖代谢改变以及无法维持钠梯度）。
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